BINC Biology Syllabus – Advanced

Cell Biology and Genetics


Vesicular transport and protein traffic in cells

Different mechanisms of signal transduction, concepts in signal network, second messenger, molecules involved in various signaling pathways such as G-protein coupled receptors, protein kinases, calcium binding proteins

X-linked and autosomal diseases, mitochondrial related disease, QTL methods for diagnostics

Extra-chromosomal inheritance

Immune response, autoimmune disorders, ELISA method

Molecular genetics and genetic disorders

Molecular Biology

Genome organization, Initiation, elongation and termination of transcription-template and enzyme properties, promoter and regulator sequences. Regulation of translation, Post transcriptional modifications

Methods for studying gene expression and regulatory sequences, large-scale expression analysis, use of microarrays

Genetic information transfer, details of regulation in eukaryotes and prokaryotes, horizontal gene transfer

Operons – positive and negative regulation, Processing of RNA and Proteins – Transport and Stability

Organization of eukaryotic genome, Methods for studying variation and polymorphism at genome level, PCR, northern, southern, western blotting, RFLP, fingerprinting, RAPDs, DNA and protein sequencing methods

Epigenetic mechanisms of inheritance regulatory RNA molecules (RNA; miRNA, siRNA), antisense RNA and their applications

Biochemistry

Enzyme kinetics, Lineweaver-Burk plot, Competitive and non competitive inhibition

Molecular mechanisms of interactions of small and large molecules including ions, regulation of protein pathways, mechanism of enzyme action, ribozyme and abzymes

Isoenzymes, allosteric enzymes, regulation by covalent modification

Carbohydrate metabolism: Glycolysis, gluconeogenesis, glycogenolysis, glycogenesis, TCA cycle and oxidative phosphorylation

Pentose phosphate pathway; hormonal control, β-oxidation and biosynthesis of fatty acids

Transamination and deamination of amino acids, ketogenic and glycogenic amino acids, urea cycle

Purine and pyrimidine biosynthesis

Bio-Informatics National Certification (BINC) Syllabus